Functional Visualization Core

image for Structural and Functional Visualization core
  • Provide state of the art imaging services to the UCLA IDDRC community, including both light and confocal microscopy; whole animal MRI acquisition and analysis support (rodent and human).
  • Development of new technologies for visualizing biological samples, focusing specifically on developing one photon miniaturized fluorescent microscopes for live imaging neural activity in situ and substrates that permit better sample clarity.
  • Supports the efforts of all the other cores, offering IDDRC researchers the ability to both probe molecular and cellular function at any level from the sub-cellular to living animals and determine the consequence of therapeutic interventions.

Services

Analysis Computer A
Computer to be used for offline image analysis (Computer A)

The Analysis Computer A is located in room 260F in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Samantha Butler at butlersj@ucla.edu or Dorwin Birt at dbirt@mednet.ucla.edu

Analysis Computer B
Computer to be used for offline image analysis (Computer B)

The Analysis Computer B is located in room 260F in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Samantha Butler at butlersj@ucla.edu or Dorwin Birt at dbirt@mednet.ucla.edu

Apotome
Structured illumination Zeiss Apotome confocal microscope Commonly used fluorophores FITC (Alexa 488), CY2, CY3, rhodamine, Texas Red, CY5 DAPI imaging possible. Axiovision image analysis. Offline analysis available. Stereo Investigator software
image for core

The Apotome is located in room 260T in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Michael Condro at mcondro@mednet.ucla.edu or Samantha Butler at butlersj@ucla.edu

Cryostat

The Cryostat is located in room 260N in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Quan Lin at qlin@mednet.ucla.edu or Samantha Butler at butlersj@ucla.edu

Evos FL Auto
The EVOS FL Auto 2 image can be used for both brightfield and fluorescence microscopy of slides, well-plates, dishes, and flasks. The on stage incubator can control the temperature, humidity, and CO2 content, as well as create a hypoxia environment. The automation software can be used to acquire z-stacks, time lapse, and tiled images. Currently, it is equipped with 4X, 10X, and 20X objectives and a DAPI, GFP, and Texas Red filter sets.

The Evos FL Auto is located in room 312 in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Samantha Butler at butlersj@ucla.edu or Michael Condro at mcondro@mednet.ucla.edu

IMaris
Imaris is Bitplane’s core scientific software module that delivers all the necessary functionality for data management, visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size, from a few megabytes to multiple gigabytes in size.

The IMaris is located in room 260F in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Samantha Butler at butlersj@ucla.edu or Dorwin Birt at dbirt@mednet.ucla.edu

Leica DMI8
Leica DMI8 inverted fluorescence microscope
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The Leica DMI8 is located in room 260S in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Dorwin Birt at dbirt@mednet.ucla.edu or Samantha Butler at butlersj@ucla.edu

LSM 800 Laser Confocal
Zeiss LSM 800 Laser Confocal Microscope

The LSM 800 Laser Confocal is located in room 260V in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Samantha Butler at butlersj@ucla.edu or Dorwin Birt at dbirt@mednet.ucla.edu

Vibratome

The Vibratome is located in room 260N in the NRB building which is located at 635 Charles Young Drive South.

For information and access please contact Quan Lin at qlin@mednet.ucla.edu or Samantha Butler at butlersj@ucla.edu